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Table 1 Primers and corresponding product sizes

From: Lack of evidence of WNT3A as a candidate gene for congenital vertebral malformations

Primer

Sequence

Length

Product size (bp)

WT-P-E1(F2)

CAGCACGTCCTCAGACACAC

20

581

WT-P-E1(R2)

CCGTCAAGGAAAACCAACC

19

 

WTE2(F)

TAGCCTGCTCATCTGTGTCG

20

367

WTE2(R)

GCTAGGCGGGAGACTCTGT

19

 

WTE3(F)

AATGGGCTAAGACCCCTGAC

20

390

WTE3(R)

GAGGCTCCTTTTTCCCAAG

19

 

WTE4(F)

CAGGCGACATGTAATGCTGT

20

644

WTE4(R)

TTTAGGTGGGAGTCCTGCTC

20

 

• WNTE3Mut(F)

ACGTTGGATG ACACGCTCATGTGCAGAAGG

30

94

• WNTE3Mut(R)

ACGTTGGATG TACAGCCACCCCACTTCCAG

30

 

• WNTE3Mut(UE)

TGTGCAGAAGGCACGGCC

18

 

• WNT-pr(F)

ACGTTGGATG TATCCGAGTGGGGCCATCGC

30

98

• WNT-pr(R)

ACGTTGGATG CGCCAGCTCCCAGGGCCCG

29

 

• WNT-pr(UE)

CCGAGAGCGTGAGCGCC

17

 
  1. The amplification and extension primers used in the homogeneous mass extend reaction are bulleted. The 10-mer tag used that increases the mass of the amplification primers so that they would fall outside the mass spectrophotometer range of analysis are bolded. UE denotes the unextended primers used in the mass extend reaction.