Structural and micro-anatomical changes in vertebrae associated with idiopathic-type spinal curvature in the curveback guppy model

Table 2 Vertebra specific two-way analysis of variance for height, thickness, and slenderness ratios

VERTEBRAL POSITION	HEIGHT RATIO			THICKNESS RATIO			SLENDERNESS RATIO
	sex	curve	sex*curve	sex	curve	sex*curve	sex	curve	sex*curve
1	0.087	< 0.0001	0.140	< 0.0001	0.0419	0.137	0.346	0.0139	0.0202
2	0.066	0.0002	0.301	0.0003	.0690	0.071	0.076	0.0482	0.161
3	0.398	0.0059	0.863	0.0014	0.183	0.142	0.0018	0.508	0.0426
4	0.495	0.0004	0.669	0.0132	0.0078	0.383	0.0053	0.260	0.0202
5	0.963	0.0004	0.489	0.0021	0.0035	0.330	0.0002	0.565	0.0160
6	0.460	0.0390	0.702	0.0280	0.185	0.737	0.457	0.965	0.0156
7	0.761	0.0038	0.948	0.0186	0.078	0.719	0.302	0.319	0.0038
8	0.734	0.0141	0.730	0.078	0.0250	0.576	0.781	0.401	0.632
9	0.151	0.750	0.645	0.0230	0.0041	0.976	0.095	0.199	0.140
10	0.299	0.0261	0.893	0.270	0.0045	0.713	0.567	0.792	0.280
11	0.0432	0.0002	0.058	0.137	0.0163	0.575	0.743	0.330	0.290
12	0.066	0.0002	0.076	0.272	0.058	0.477	0.150	0.533	0.595
13	0.312	0.0015	0.708	0.291	0.0265	0.995	0.130	0.150	0.099
14	0.0393	0.0482	0.689	0.217	0.212	0.984	0.746	0.808	0.782
15	0.497	0.873	0.260	0.104	0.0309	0.857	0.877	0.233	0.866

Significant interactions (sex*vertebral position and vertebral position*curvature) for the height ratio (Figure 4) motivated us to investigate each vertebra using a two-way ANOVA. Also, because the thickness and slenderness ratios had a significant sex*curvature interaction, they were further investigated using a two-way ANOVA on data for each vertebra. Significant results are shown in bold, (significance threshold: α = 0.05).

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