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Volume 10 Supplement 1

IRSSD 2014 Meeting Abstracts

  • Oral presentation
  • Open Access

Is sympathetic nervous system involved in the etiopathogenesis of idiopathic scoliosis? - A preliminary animal study in bipedal C57BL/6J mice model

  • Zhen Liu1,
  • Jing Guo1,
  • Tao Wu1,
  • Bangping Qian1,
  • Zezhang Zhu1,
  • Feng Zhu1,
  • Jian Jiang1 and
  • Yong Qiu1
Scoliosis201510(Suppl 1):O12

https://doi.org/10.1186/1748-7161-10-S1-O12

Published: 19 January 2015

Keywords

Bone Mineral DensityPropranololSympathetic Nervous SystemAdolescent Idiopathic ScoliosisLower Bone Mineral Density

Summary

A preliminary animal study to investigate whether the incidence of scoliosis or the magnitude of curves can be changed by pharmacological sympathectomy in bipedal C57BL/6J mice model.

Introduction

The sympathetic nervous system (SNS), as an important part of autonomic nervous system, through its hypothalamic neuroendocrine control of puberty, menarche and skeletal growth may contribute significantly to the pathogenesis of adolescent idiopathic scoliosis (AIS). Hyperactivity of the SNS has been reported to result in lower body mass index (BMI), lower bone mineral density (BMD), and longer extremities, which are commonly seen in AIS patients. The objective of this study was to investigate the effect of sympathectomy by way of pharmacological agents on the development of scoliosis in bipedal mice model.

Methods

Sixty female 3-week-old C57BL/6J mice were divided into three groups after amputations of forelimbs and tails. Group 1 received daily intraperitoneal injection of 0.9% saline (5 ml/kg/day); while Group 2 and 3 received sympathectomy by daily intraperitoneal injection of propranolol (20 mg/kg/day) and guanethidine sulfate (40 mg/kg/day), respectively. Posteroanterior X-rays were obtained at 20th week. Curves were measured using Cobb method and scoliosis was defined as a Cobb angle of > 10°. Incidence of scoliosis and severity of curves were compared among groups.

Results

There were 17 (85%) mice presented scoliosis in Group 1; whereas 11 (55%) and 10 (50%) mice presented scoliosis in Group 2 and 3, respectively. The incidence of scoliosis was found to be higher in Group 1, but the statistical significance was just marginal (P=0.046). As for curve magnitudes, the mean Cobb angle was 20.5°±9.2° in Group 1, 10.2°±6.8° in Group 2, and 11.7°±7.9° in Group 3. The mean Cobb angle of Group 1 was significantly greater than those of Group 2 and 3 (P<0.01), but there was no significant difference in mean Cobb angle between Group 2 and 3.

Conclusion

Sympathetic nervous system may be involved in the development and progression of scoliosis in bipedal C57BL/6J mice model. Sympathectomy do not seem to dramatically decrease the incidence of scoliosis, probably due to that bipedalism itself may also be a cause of scoliosis in this animal model.

Authors’ Affiliations

(1)
Spine Surgery, the Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, China

Copyright

© Liu et al; licensee BioMed Central Ltd. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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