- Poster presentation
- Open access
- Published:
Comparison of proliferation and differentiation of osteoblasts derived from different locations in patients with adolescent idiopathic scoliosis
Scoliosis volume 10, Article number: P4 (2015)
Objective
To compare the proliferation and differentiation of osteoblasts derived from different locations in adolescent idiopathic scoliosis (AIS) patients.
Methods
From January 2011 to May 2012, a total of 16 AIS patients (14 females, 2 males) were recruited in the study, with age from 11 to 17 years. All patients received posterior spinal fusion and instrumentation, as well as thoracoplasty. Trabecular bone chips from ribs, lamina and iliac crest were harvested in each patient intraoperatively for osteoblast culture. P2 cells were used for the experiment. Proliferation and differentiation of osteoblasts were investigated by using MTT method and alkaline phosphatase (ALP) activity, respectively. One-way analysis of variance (ANOVA) was used for comparison of proliferation and differentiation among osteoblasts derived from different locations.
Results
For MTT assay, the proliferation of lamina osteoblast (OD value 0.276±0.125) was slightly lower, whereas the proliferation of rib osteoblast (OD value 0.319±0.117) was slightly higher, than that of ilium osteoblast (OD value 0.294±0.141); however, there was no statistical difference among the 3 types of osteoblasts (P>0.05). ALP activity assay showed that the differentiation of both lamina (OD value 0.308±0.129) and rib (OD value 0.293±0.114) osteoblasts were slightly higher than that of ilium osteoblast (OD value 0.281±0.102), but without statistical significance (P>0.05).
Conclusion
There was no difference in the proliferation and differentiation of osteoblasts derived from rib, spinal lamina and iliac crest in patients with AIS. The results of the current study indicate that local and rib bones harvested during surgery may have comparable osteogenic activities to the iliac bone grafts for spinal fusion in AIS patients, potentially eliminating the need for harvesting autologous iliac bone.
Author information
Authors and Affiliations
Rights and permissions
Open Access  This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made.
The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.
To view a copy of this licence, visit https://creativecommons.org/licenses/by/4.0/.
The Creative Commons Public Domain Dedication waiver (https://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
About this article
Cite this article
Guo, J., Lam, Tp., Lee, S. et al. Comparison of proliferation and differentiation of osteoblasts derived from different locations in patients with adolescent idiopathic scoliosis. Scoliosis 10 (Suppl 1), P4 (2015). https://doi.org/10.1186/1748-7161-10-S1-P4
Published:
DOI: https://doi.org/10.1186/1748-7161-10-S1-P4