Volume 9 Supplement 1

11th International Conference on Conservative Management of Spinal Deformities - SOSORT 2014 Annual Meeting

Open Access

Hystological paraspinal muscle comparison between curve concavity and convexity in adolescent idiopathic scoliosis

  • Marcelo Wajchenberg1,
  • Rafael Paiva Luciano1,
  • Delio Eulalio Martins1,
  • Luciano Miler Reis Rodrigues2,
  • Eduardo Barros Puertas1,
  • Moises Cohen1,
  • David Del Curto1,
  • Acary Souza Bulle de Oliveira1 and
  • Beny Schmidt1
Scoliosis20149(Suppl 1):O12

https://doi.org/10.1186/1748-7161-9-S1-O12

Published: 4 December 2014

Background

Several studies have presented morphological, biochemical and histopathological changes in the paraspinal skeletal muscle of patients with adolescent idiopathic scoliosis (AIS). Some abnormalities have been demonstrated such as an increased amount of type I fibers in the concavity and the decreased number of type II in the convexity, an increase in the intracellular concentration of glycogen and lipids, structural changes in the sarcolemma and myotendinous junction, changes in the activity muscle enzyme, an increase in intracellular calcium concentration.

Aim

To evaluate the rotator muscle fibers at the apical vertebraof curves of patients with AIS by histological and immunohistochemical analysis.

Design

Cross-sectional study.

Methods

Twenty-one patients with AIS submitted to surgical correction between 2,010 and 2,013 had the spinal rotator muscles biopsied in the concavity and convexity of the apical vertebra of the thoracic curve during the surgery. Serial cryosections were stained with Hematoxylin-Eosin (HE) and Sudan Red. We evaluated muscle atrophy and hypertrophy, fatty proliferation, presence of endomysial and perimysial fibrosis, presence of hyaline fibers, mitochondrial proliferation, muscle necrosis, nuclear centralization and inflammation. Two independent observers evaluated the sections.

Results

The average value of the angle of the main thoracic curve was 68° Cobb. For analysis of non-parametric paired variables between the concave and convex side it was used the McNemar test with a significance level of 5%. The results of relative frequency and “p” values showed significant difference in both endomysial and perimysialfibrosis and fatty involution when compared to both sides of the apical vertebra.

Conclusion

The paraspinal muscles at the apex of the deformity had higher involvement in the concavity when analyzing the fatty involution and fibrosis. However, both sides showed signs of myopathy, muscle atrophy through areas of necrosis and hyaline presence of fibers, mitochondrial proliferation.

Authors’ Affiliations

(1)
Federal University of Sao Paulo
(2)
ABC University

Copyright

© Wajchenberg et al; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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