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Structural and micro-anatomical changes in vertebrae associated with idiopathic-type spinal curvature in the curveback guppy model
© Gorman et al; licensee BioMed Central Ltd. 2010
Received: 17 March 2010
Accepted: 7 June 2010
Published: 7 June 2010
The curveback lineage of guppy is characterized by heritable idiopathic-type spinal curvature that develops during growth. Prior work has revealed several important developmental similarities to the human idiopathic scoliosis (IS) syndrome. In this study we investigate structural and histological aspects of the vertebrae that are associated with spinal curvature in the curveback guppy and test for sexual dimorphism that might explain a female bias for severe curve magnitudes in the population.
Vertebrae were studied from whole-mount skeletal specimens of curved and non-curved adult males and females. A series of ratios were used to characterize structural aspects of each vertebra. A three-way analysis of variance tested for effects of sex, curvature, vertebral position along the spine, and all 2-way interactions (i.e., sex and curvature, sex and vertebra position, and vertebra position and curvature). Histological analyses were used to characterize micro-architectural changes in affected vertebrae and the intervertebral region.
In curveback, vertebrae that are associated with curvature demonstrate asymmetric shape distortion, migration of the intervertebral ligament, and vertebral thickening on the concave side of curvature. There is sexual dimorphism among curved individuals such that for several vertebrae, females have more slender vertebrae than do males. Also, in the region of the spine where lordosis typically occurs, curved and non-curved females have a reduced width at the middle of their vertebrae, relative to males.
Based on similarities to human spinal curvatures and to animals with induced curves, the concave-convex biases described in the guppy suggest that there is a mechanical component to curve pathogenesis in curveback. Because idiopathic-type curvature in curveback is primarily a sagittal deformity, it is structurally more similar to Scheuermann kyphosis than IS. Anatomical differences between teleosts and humans make direct biomechanical comparisons difficult. However, study of basic biological systems involved in idiopathic-type spinal curvature in curveback may provide insight into the relationship between a predisposing aetiology, growth, and biomechanics. Further work is needed to clarify whether observed sex differences in vertebral characteristics are related to the female bias for severe curves that is observed in the population.
The curveback lineage of the guppy, Poecilia reticulata is characterized by heritable idiopathic-type spinal curvature that progresses during growth in otherwise healthy individuals. Curveback is derived from a single cross between a curved male and an unrelated non-curved female, and consists of curved and non-curved individuals . Curved individuals display deviation of the spine primarily in the sagittal plane of the body as an anterior lordosis and posterior kyphosis in the tail (non-rib associated vertebrae) . In addition to having a strong genetic component, prior study of the curveback phenotype has demonstrated several developmental similarities to the human idiopathic-type scoliosis (IS) syndrome. These include: curve onset after birth (guppies give birth to live fry), curve stabilization at sexual maturity, phenotypic variability (for age of curve onset, curve magnitude, and the propensity for a curve to stabilize or progress to severity), and a greater likelihood for females to develop severe curvature . These parallels suggest that curveback is a feasible animal model for exploring the biological systems involved in heritable idiopathic-type spinal curvature. However, better characterization of the curveback phenotype is necessary to understand how the model might be applied to specific research questions.
Characteristically, spinal curvature in curveback lacks congenital anomalies (e.g. failure of formation or segmentation), although distortion of vertebral shape at and around the apex has been noted . In order to characterize vertebral shape distortion associated with curvature in curveback, we compare structural and micro-anatomical differences among the vertebrae of curved and non-curved adults. The results are then related to studies regarding human spinal curvatures as well as studies involving vertebral distortion in animals.
The guppy P. reticulata is a small, live-bearing teleost fish that is native to the streams of northeast South America. The species has been a model organism for ecological, evolutionary, developmental, and genetic research since the 1920's. The curveback lineage is composed of curved and non-curved individuals derived from a laboratory population that has been maintained by inbreeding since 2003. The lineage consists of multiple inbred families, all propagated from a single curved male. Laboratory fish are kept under standardized conditions  in compliance with protocols approved by the Simon Fraser University Animal Care Facility and the Canadian Council on Animal Care.
Whole-mount skeletal staining
Twenty-seven adult fish (14 females: 6 curved, 8 non-curved; 13 males: 6 curved, 7 non-curved) were stained for bone and cartilage. All fish were euthanized at a minimum of 3 months past birth and then fixed in 10% neutral-buffered formalin (Sigma). Specimens were fixed for 2 days, and then washed in several changes of distilled, deionized water (ddH2O) (>1 hour each), then dehydrated in 50% ethanol (EtOH) (24 h) and then 100% EtOH (24 h). Whole fish were then incubated in cartilage staining solution (8GX Alcian blue in 70% anhydrous EtOH and 30% acetic acid) for 24 hours at room temperature with mild agitation. Stained specimens were neutralized in saturated aqueous sodium borate solution for 9-12 hours and then bleached for 20 minutes (3% hydrogen peroxide and 1% potassium hydroxide). Specimens were then digested with a trypsin solution (1 g trypsin in 35 ml saturated sodium borate/65 ml ddH2O) until they were 60% clear. Bones were stained with Alizarin red solution in 0.1% potassium hydroxide solution at room temperature with mild agitation, until they were light red in colour. Specimens were destained in trypsin for 40-48 hours and then preserved in stepwise solutions of 30%glycerol/70% of 1% KOH, 60% glycerol, and 100% glycerol. Thymol crystals were added to glycerol to prevent fungal growth during long-term storage of specimens.
Whole-mount stained specimens were positioned on their side on a glass slide and viewed through a Meiji MEI (Tokyo, Japan) stereoscope under 4× and 2× magnification. Photographs were taken with a Pentax MX camera though a MA150/T2 (Meiji) adapter at 2× magnification. All photographs were taken so that the camera looked down on the profile of the fish. Measurements were taken from digitized photos (ImageJ software, http://rsbweb.nih.gov/ij/). Statistical analysis was conducted using JMP software for Mac, Version 7.0 (SAS Institute, INC., Cary, NC, USA).
Tails from 4 curved and 2 non-curved female curveback guppies were fixed overnight in 10% neutral-buffered formalin and then demineralized in a solution of 10% formaldehyde: 5% ethylenediaminetetraacetic acid (EDTA) for a minimum of 2 weeks. For half of the curveback specimens, tail vertebrae 4 and 11, which occur at the apex of the lordosis and kyphosis, respectively, were carefully dissected under a stereomicroscope. This was to ensure that they could later be precisely embedded for sectioning in the transverse plane. Tails to be sectioned in the sagittal plane were left intact.
Following several rinses in 1× phosphate-buffered saline (PBS), body segments were dehydrated through an ethanol: PBS series, cleared in xylene, and penetrated with paraffin under vacuum. Specimens were embedded under a stereomicroscope and sectioned in either the transverse or sagittal planes at a thickness of 7-10 μm. Optimal sectioning generally required that the blocks were first 'softened' by soaking in distilled water.
Tissue sections were stained with either Mallory's trichrome (as per ) or double-stained with Picrosirius Red and Alcian Blue (as per ). By the former protocol, collagen fibrils are stained blue. By the latter, collagen type I and bone appear red and sulfated proteoglycans, which are enriched in cartilage, as blue. Sections were photographed using a Hitachi HV-F22 3-CCD digital camera coupled to a Zeiss Axioskop compound microscope.
Vertebral Shape Analysis
For each ratio, individual effects from sex, curvature, position of vertebra in tail, and their interactions (sex*vertebra position, sex*curvature, vertebra position*curvature) were tested using a fixed-effects model ANOVA (three-way analysis of variance) (significance threshold: α = 0.05). Due to the fact the sample size for this study is small, we do not expect these tests to have statistical power sufficient for conclusive determinants of shape changes. Rather, our heuristic analysis uses ANOVA to compare means relative to variability within groups in order to detect general trends in vertebral shape.
For all ratios, vertebra position had a considerable effect. In order to identify which vertebrae were significantly contributing to these results, we separated the effects of vertebra position by conducting separate ANOVAs on each vertebra. Ratios with significant effects for either sex or curvature, but no sex*curvature interaction, were further investigated using one-way ANOVAs for either sex or curvature on data for each individual vertebra. Ratios with a significant interaction effect of sex and curvature were further investigated using a two-way ANOVA on data for each vertebra. For all data, the distribution of values was tested for normality using Shapiro-Wilks goodness-of-fit (α = 0.05). Statistical analyses were conducted using JMP software for Mac, Version 7.0 (SAS Institute, INC., Cary, NC, USA).
The axial skeleton of P. reticulata
Gross morphological changes in vertebrae associated with spinal curvature in curveback
Vertebra specific one-way analysis of variance for length ratio and width ratios.
Vertebra specific two-way analysis of variance for height, thickness, and slenderness ratios
Because there was a significant interaction between sex and vertebra position (p = 0.0371) for the width ratio, we further investigated the width ratio among individual vertebrae for males and females. The results show that the mean values for vertebrae 7 and 10 are significantly higher in females compared to males (Table 1- vertebra 7: 1.03+/-0.02 S.E. compared to 0.97, +/- 0.02 S.E., respectively; vertebra 10: 1.06, 0.02SE to 0.99, 0.02SE). To determine whether the vertebrae are actually wedged, we tested whether the mean values for curved males and females are significantly different from one (t-test, α = 0.05), which they were not.
The height ratio reflects the greatest change in vertebral shape that is associated with curvature, with significant effects from sex and curvature. Significant interactions (sex*vertebral position and vertebral position*curvature) motivated us to investigate each vertebra using a two-way ANOVA (Table 2). Among curved males and females, the normally amphicoelous shape of tail vertebrae 1-8 is distorted such that the height is increased on the dorsal side and reduced on the ventral side. For vertebrae 10-14 the height is increased on the ventral side and reduced on the dorsal side. The increase in dorsal height corresponds with lordosis, while the increase in ventral height with kyphosis. For vertebrae 11 and 14, this kyphotic distortion is more severe among females. Vertebrae 9 and 15 do not show significant distortion of the amphicoelous shape (Table 2, Figure 5).
The thickness ratio varied significantly with sex, and the slenderness ratio with curvature, and both ratios had a significant sex*curvature interaction (Figure 4). Therefore, these ratios were further investigated using a two-way ANOVA on data for each vertebra. The thickness ratio describes the width of the middle of the vertebral centrum, relative to the posterior width. Among non-curved and curved individuals, the vertebral thickness is sexually dimorphic such that among females the midline width is reduced for vertebrae 1-9. Among curved males and females, the midline width is reduced for vertebrae 1, 4, 5, 8-11, 13, 15 (Figure 5). The slenderness ratio describes the length of a vertebra relative to its width. Among curved individuals, the first three tail vertebrae have a reduced length relative to their width (compared to non-curved individuals). For vertebrae 4-7, among curved individuals, values for the slenderness ratio were sexually dimorphic such that vertebrae were more slender among females (Table 2, Figure 5).
Micro-anatomical changes in lordotic and kyphotic curveback vertebrae
The most pronounced defects associated with idiopathic-type curvature in the curveback guppy are in intervertebral ligaments occurring at the approximate apex of curvature (Figure 7B, 7F). For these ligaments, the fibrous layer appears to migrate towards the convex face of the spinal curvature in response to compressive forces acting on the concave side of the ligament (Figure 7B', 7F'). This displacement of the inner fibrous layer causes compression of the outer spongy layer on the convex face and distension on the opposite side. Thus, in intervertebral discs occurring at the apex of a lordotic curve, the inner fibrous layer migrates ventrally, while the spongy outer layer becomes distended dorsally (Figure 7B, 7B'). Conversely, in kyphotic curves, the fibrous layer is displaced dorsally and the spongy outer layer distended ventrally (Figure 7F, 7F').
Structural and micro-anatomical changes associated with curvature in the curveback guppy
With lordosis, the concave side of the curve is dorsally directed, and with kyphosis the concavity is ventrally directed. Generally, we found that morphological change associated with lordosis and kyphosis is suggestive of anterior-posterior (i.e. cranial-caudal) compression along the spine. Structural evidence for this is from whole mount staining of skeletons showing that the normal amphicoelous (hour-glass) shape of vertebrae is distorted so that vertebral height is reduced on the convex and is greater on the concave side of curvature. In addition, vertebrae at the approximate apex of curvature (lordotic vertebra number 6 and kyphotic vertebrae 10 and 11) are wedged so that the length on the concave side of the curve is reduced relative to the convex length. Also, the midline width is significantly reduced for some vertebrae. Histological evidence for cranial-caudal compression is from micro-anatomical changes in both vertebrae and intervertebral ligaments (Figure 7). In curved guppies the centra and vertebral arch bases appear thicker on the side of vertebrae facing the concavity of a curve, and the spongy layer of the intervertebral ligament is displaced towards the convexity of the curve (Figure 7B, 7F), The observed changes in vertebral bone structure may be due to either (1) distortion of normal vertebral shape or (2) active remodelling of vertebral osteoid bone as a consequence of extrinsic forces. The latter phenomenon has been described in animal models with induced curvature [20–22], in the facets of patients with IS [23, 24], as well as in a number of teleost species [12, 14, 25–28]. Further study in curveback should test whether vertebrae are undergoing active osteoid remodelling by volumetric analysis such as that employed by Kranenbarg and others [14, 28].
There are similar concave-convex biases that occur along the cranial-caudal axis in the curveback and human and induced mammalian model curve phenotypes such as vertebral shape distortion, thickening of the bone, and intervertebral disc migration. With human idiopathic-type curvatures such as IS and Scheuermann kyphosis, as well as with curves associated with syndromes such as cerebral palsy, 3-M syndrome, and achondroplasia there is abnormal vertebral wedging (defined by a reduction in vertebral length on the concave side of a curve, with no significant length changes on the convex side) at and around the apex of curvature [29–36]. Vertebral wedging in human syndromes is often explained by the Hueter-Volkmann principle, which states that vertebral body growth is retarded by continuous or excessive mechanical compression upon the epiphysis (and stimulated by reduced compression) [37, 38]. Distortion of vertebral shape consistent with the Hueter-Volkmann principle has been induced in animal models by direct mechanical stress (dog , monkey , calf tail , rat-tail [42, 43], goat [44–46], rabbit , rat and rabbit and calf ). With induced models, asymmetrically loaded vertebrae demonstrate changes in growth rate along their growth plates, causing uneven progression in longitudinal growth and consequential shape distortion in the form of wedging . In contrast to other animal models, the guppy has vertebrae that are composed of acellular bone (i.e. devoid of embedded osteocytes and formed by intramembranous ossification) (reviewed in ). Therefore further study of vertebral wedging in curveback should test cellular activity at the intervertebral region, the presumed growth zone of guppy vertebrae , to assess whether there is modulation of growth in curved individuals.
Recently, Driscoll and others  investigated the health of the intervertebral disc, migration of the nucleus pulposus, and trabecular bone mineral density (BMD) as indicators of concave-convex stresses associated with curvature in IS. In a finite element model, they demonstrated that increased BMD, annular degeneration, and disc migration played a moderate role in curve progression because they altered the path of force transmission within the spine. Considering that in curveback, there are concave-convex biases similar to those associated with IS [i.e. vertebral shape distortion, thicker bone on the concave side of curvature, displacement of the spongy layer of the intervertebral ligament (similar to the mammalian nucleus pulposus) toward the convexity of curvature] they may also contribute to curve progression in the guppy by altering the path of force transmission.
Sexual dimorphism associated with spinal curvature
In human studies, the ratio of vertebral height/width has been used as an index of spinal slenderness, and females are reported to have more slender vertebrae than boys [5–9]. It has been suggested that sexual dimorphism for vertebral slenderness might be related to the greater likelihood for females with IS to develop curves of higher magnitude, because slenderness would reduce vertebral column stability and thus increase the susceptibility for a spine to buckle . As in humans, the guppy curve phenotype is suggestive of column buckling, and the guppy vertebral column is subject to cranial-caudal loading (see ). Because in curveback there is a female bias for severe curves, we also compared the relative slenderness of vertebrae among males and females from the curveback lineage.
Among curved guppies, females tend to have more slender vertebrae in what appears to be the primary curve (lordosis). Vertebrae numbers 4-7 are more slender among curved females compared to non-curved males, non-curved females, and curved males (length of vertebrae relative to width). These results are similar to that described by Skogland and Miller (1981), who suggest that only a part of the vertebral column is more slender in girls with IS. It is important to note however, that investigations into the relationship between vertebral body slenderness, gender, and IS have given inconsistent results due to differences in sample size and constitution (e.g., age), as well as whether one or multiple vertebrae were measured (see: [6–8, 50]. Therefore, whether sexual dimorphism for vertebral slenderness is related to the observed female bias for severe curves in idiopathic-type scoliosis in humans is unclear.
We also found that among non-curved females of the curveback lineage, the first nine tail vertebrae have a relatively thinner midline width compared to non-curved males. A thicker midline width among non-curved males might be related to physiological and biomechanical factors associated with the male gonopodium and its suspensorium. Among the family Poeciliidae, the anal fin and vertebrae involved in its suspension (tail vertebrae 1 and 2) are sexually dimorphic, so that in mature males, the anal fin is transformed into the gonopodium (an intromittent organ) and the first two tail vertebrae form its suspensorium (see Figure 3) . Among guppies, the length of the gonopodium can reach 50% of the total body length. In addition, as a part of courtship activities, the male guppy engages in extensive flicking, and swinging back and forth of the gonopodium. These activities have the potential to put sex-specific biomechanical strain on the supporting skeletal structure that could increase midline thickness. Therefore, further study is necessary to determine whether vertebral thinness is a predisposing risk factor for a greater curve magnitude among curveback females. Future studies should also examine the vertebrae of individuals who are not from the curveback lineage (i.e. lineages that do not exhibit spinal curvature).
Curveback and its relation to spinal curvatures in humans
The curveback phenotype is primarily a sagittal plane deformity and so although previous studies have identified developmental parallels to IS [1, 51], structurally the phenotype appears to be more similar to Scheuermann kyphosis. A limitation in our capacity to discuss comparisons between curveback and Scheuermann kyphosis is the lack of available studies regarding its natural history (reviewed in ). As a consequence, much of our comparisons to human studies discussed here relate to IS. Although there are extensive genetic and physiological similarities between teleosts and mammals, anatomical differences make direct biomechanical comparisons difficult. For example, the teleost spine is not weight bearing due to the buoyancy from the swim bladder and the density of water . That most human spinal curvatures are manifest in the coronal plane and the curveback deformity is in the sagittal plane may reflect the different muscle and ligament organization and consequently different tensions on the spine. Indeed, in response to pinealectomy, chickens demonstrate 3-dimensional spinal curvature with vertebral wedging , but salmon demonstrate spinal curvature in the sagittal plane only .
With regard to idiopathic-type spinal curvature, the relationship between predisposing factors, growth, and mechanical stress is unknown. Although the curveback guppy is an appropriate model for understanding the basic biological systems involved in idiopathic-type curvature, its use for biomechanical study that directly relates to humans is limited. However, once molecular mechanisms involved in idiopathic-type spinal curvature are defined, it will be important to understand them in the context of growth and biomechanics, and therefore exploration of structural aspects of curveback curvature is important.
Based on similarities to human spinal curvatures and to animals with induced curves, the concave-convex biases described in the guppy suggest that the progression of curvature during growth is biomechanically mediated in curveback. However, this study does not allow us to discern whether the vertebral anomalies described in curveback are primary or secondary to the curve. Further studies with in-vivo imaging technology may allow for a better understanding of how vertebral shape changes relate to curvature during growth. Further experimentation should also investigate the sexual dimorphisms reported in this study in order to test for an association with severe curves among females in the curveback population.
We acknowledge Dr. Julian Christians, Dr. Ian Stokes and Dr. Clayton Adam for their insights and suggestions. We are thankful to Dr. Wainwright for his clear and stain protocol, which we adapted for guppies. We are thankful to Dr. S. Tredwell for his input and interest. This study received support from the Scoliosis Research Society, Natural Sciences and Engineering Research Council of Canada, and the US National Institute of Health (#1R21AR053730-01A2). The SFU University Animal Care Committee, project #763B, approved this research.
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